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qPCR Kits

Real-Time PCR Kits

Real-Time PCR Kits

Compatible with all Real Time PCR platforms                                      

 

Radiant Real-time Mix

Alkali Scientific offers Radiant™ qPCR Kits, a high-performance portfolio of 2X ready-to-use qPCR mixes designed for best-in-class real-time PCR.  Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to significant improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays. Radiant™ qPCR Kits are available for intercalating-dye based assays (GREEN) and also hydrolysis probe assays (TaqMan® and molecular beacons).

  • Novel hot-start chemistry for improved specificity and sensitivity.
  • Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed.
  • Broad Range detetion for increased reliability in low-copy assays.
  • Versatile 2X Kits, suitable for both fast and standard cycling methods.

      SYBR Green qPCR Kits     Probe Assay qPCR mastermix              
qpcr reagent sample


Intercalating-Dye Assays (SYBR Green)      

Radiant™ SYBR Green qPCR kits are designed for the majority of applications for the fast, sensitive detection of cDNA, genomic DNA and viral targets. Radiant™ SYBR Green qPCR kits include a pre-mixed, proprietary, non-inhibitory DNA-binding dye which also provides the option of a post amplification melt profile.

Comparative data on qPCR kits

Comparative amplification and melt traces of two mouse housekeeping genes (ACTG1 and CANX respectively) using Radiant™ Green Lo-Rox qPCR Kit (purple) and ABI Power SYBR Green Mix (black). Please note the significantly lower Ct values and overal superior performance of Radiant™ Green Lo-Rox qPCR Kit.


 
1-Step SYBR Green
 
Radiant™ 1-Step SYBR Green Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Radiant™ Taq HS DNA Polymerase, resulting in robust RT-PCR performance.
 
Radiant™ 1-Step SYBR Green Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency.  Proprietary small molecular inhibitor technology preventys formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polyerase tehnology and advanced buffer chemistry, we offer market leading performance with minimal or no optimization.

 

      SYBR Green qPCR Kits     Probe Assay qPCR mastermix      


Probe Assays      

Radiant™ Probe qPCR Kits are best-in-class kits for hydrolysis probe assays (TaqMan®, Molecular Beacons® and Scorpion probes®.) The primary advantage of probe based technologies is multiplex capability (in which multiple amplicons can be detected in the same reaction). Radiant™ Probe qPCR Kits have been developed for market-leading sensitivity across a wide variety of templates, in singleplex as well as multiplex PCR reactions.

Real-Time PCR Kits Comparative Data

TaqMan® Probe amplification traces of human gene ACVR2B in singleplex and quadplex (ACVR2B, LIMK1, ACVR1B, and CDK7) from a cDNA serial dilution series. Panel 1 (Radiant™ Probe No-Rox qPCR Kit) shows significantly less PCR inhibition in quadplex than Panel 2 (Bio-Rad® Sso Fast Probe Mix).

 



1-Step Probe Assays

Radiant™ Probe 1-Step qPCR Kits provide the convenience, optimal work-flow, and sample integrity for highly efficient cDNA synthesis and qPCR in a single tube. The kits can be used universally, across all RNA templates such as total RNA, mRNA and viral sequences and utilize a highly optimized, thermostable reverse transcriptase in combination with advances in buffer chemistry for superior sensitivity, reproducibility and multiplex capability.


      SYBR Green qPCR Kits     Probe Assay qPCR mastermix      


We're confident Radiant™ qPCR Kits will work for you!

Call us for a free sample. 1-888-927-5629
 
rt-pcr kits

  


 

Have questions or want to try a sample? Contact us at 1-888-927-5629. Our friendly customer service team is here to help you find the right qPCR kit for your machine and research. We offer a variety of kits for the most popular real-time PCR machines.