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Cell Proliferation WST-1 Reagent

• Stability 18 months
• Storage -20°C
• Shipping - Blue ice in continental US; may vary elsewhere

Availability: In stock

Product Name Cat # Price Qty
Cell Proliferation WST-1 Reagent, 1.0 ml for up to 100 tests Y301
$19.95
Cell Proliferation WST-1 Reagent, 8 ml for up to 800 tests Y410
$109.95
Cell Proliferation WST-1 Reagent, 25 ml for up to 2500 tests Y525
$249.95
 

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Product Description

Details

The Cell Proliferation Reagent WST-1 is a ready-to-use colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates. Quickly and accurately measure mitochondrial health Far quicker and more efficient than an MTT assay Typical Use: Primary screening counterstain in a Cell Biology environment Convenient: Benefit from a ready-to-use reagent. Safe and Easy: Eliminate radioactive isotopes, washing steps, and additional reagents. Accurate: The absorbance obtained strongly correlates to the cell number. Sensitive: Detect low cell numbers. Fast: Process a large number of samples using a multi-well ELISA reader. Contents Ready-to-use solution, containing WST-1 and an electron coupling reagent. Available in the following sizes : 0.5ml 8.0ml 25.0ml DETAILS & HOW IT WORKS: Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate. The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture. Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells. Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells
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