Lambda Phage DNA, Methylated from Escherichia coli host strain W3110, 1 X 1 unit (D3779-1UN)

Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This methylated lambda DNA is not completely digested by Bcl I, Cla I, Mbo I, Mbo II, Taq I or Xba I whereas non-methylated lambda DNA(product number D3654) is only partially cleaved. Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 has been used: •in magnetic tweezers measurements •in the preparation of ³⁵S-labeled DNA •in the preparation of coupled standards (DNA + RNA) DNA is supplied in a solution of 10 mM Tris-HCl (pH 8.0), with 1mM EDTA. Unique restriction sites: Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I. Methylated lambda is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I. One O.D. (A₂₆₀) is approximately 50 μg of DNA

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