PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6), 100 µG (676604-100UG)

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷. ELISA (0.05 µg/ml) Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells) Lyophilized from PBS, PEG, sucrose. Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem.275, 40. Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723. Human Positive Control Vanadate-treated A431 cells Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Toxicity: Standard Handling (A)

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