CellPro™ Hank’s Balanced Salt Solution (HBSS) 1X without calcium and magnesium is a high-quality, isotonic buffer formulated to support a wide range of cell handling and washing applications. By removing calcium and magnesium ions, this HBSS formulation helps minimize cell–cell and cell–surface adhesion, making it ideal for procedures requiring gentle cell dissociation, suspension, or recovery.
This ready-to-use solution maintains physiological pH and osmolarity, ensuring cell integrity during processing outside of complete culture media. It is commonly used for tissue dissociation workflows, cell washing steps, reagent preparation, and transport of cells or tissues.
Key Features & Benefits
- Calcium- and magnesium-free formulation reduces unwanted cell aggregation and adhesion
- Isotonic, balanced salt solution that preserves cell viability during handling
- Ideal for cell washing, suspension preparation, and reagent dilution
- Supports enzymatic dissociation by preventing cation-dependent cell interactions
- Low endotoxin, high-purity components
- Ready-to-use 1X format for convenience and consistency
Applications
- Cell washing and centrifugation steps
- Preparation of single-cell suspensions
- Use with trypsin or other dissociation enzymes
- Tissue handling, transport, and processing
- Buffering during immunology, microbiology, and molecular biology workflows
