Norgen Biotek™ Clone Sizer 100bp DNA Ladder, 100 Loads

Norgen Biotek Corp.™

SKU:

11600

$75.00

Net 30 Terms Available

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Ready-to-use Quantitative Highly Stable Precise Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments Higher intensity reference bands at 500 bp and 1000 bp 2686 bp (pUC19) reference band for easy clone identification The CloneSizer 100 bp DNA Ladder (11600) is prepared to ensure quality…

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CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads

Ladder Properties:

Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
Higher intensity reference bands at 500 bp and 1000 bp
2686 bp (pUC19) reference band for easy clone identification

Fragment	Size (bp)	Mass (ng)
1	2686	72
2	2000	53
3	1500	41
4	1200	42
5	1000	56
6	900	30
7	800	29
8	700	25
9	600	25
10	500	52
11	400	19
12	300	20
13	200	19
14	100	17

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.