Norgen Biotek™ DNA Clean-Up and Concentration Micro-Elute Spin Column Kit, 50 Preps

Norgen’s Purification Technology 

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first mixing the DNA samples or enzymatic reactions containing DNA with Buffer RL. Ethanol is then added and the mixture is loaded onto an activated spin-column specifically designed for small elution volumes. Norgen’s resin binds DNA in a manner that depends on ionic concentrations.  Thus only the DNA will bind to the column, while the contaminating proteins or free nucleotides will be removed in the flowthrough.  The bound DNA is then washed three times with the provided Wash Solution A in order to remove any remaining impurities.  The purified DNA is then eluted into a small volume (8 –15 μL) using Elution Buffer F.

Advantages

• Concentration of small amounts of DNA into 8 to 15 μL
• Ideal for concentrating DNA from PCRs and other enzymatic labeling reactions
• No need for organic denaturants or chloroform
• Cleanup of plasmids or DNA isolated using other DNA isolation methods
• Fast and easy processing using rapid spin-column format 
• Suitable for DNA from 50 bp to 10,000 bp