Norgen Biotek™ FFPE DNA Purification Spin Column Kit Dx, 50 Preps

SKU:

Dx47400

$269.00

Net 30 Terms Available

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Fast and easy processing using rapid and convenient spin-columns Isolate high quality and high yield DNA DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping Norgen’s FFPE DNA Purification Kit Dx (Dx47400) p…

Fast and easy processing using rapid and convenient spin-columns
Isolate high quality and high yield DNA
DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping

Norgen’s FFPE DNA Purification Kit Dx (Dx47400) provides a rapid method for the isolation and purification of genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples for subsequent in vitro diagnostic use. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the DNA over time. Norgen’s FFPE DNA Purification Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of DNA. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components without the use of phenol or chloroform.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s FFPE DNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s FFPE DNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s FFPE DNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

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Kit Specifications
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Loading Volume Per Spin Column	650 μL
Size of DNA Purified	All sizes > 80 bp
Maximum Amount of Starting Material	5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K and RNase should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. Dx47400 (50 preps)
Digestion Buffer	20 mL
Binding Solution	20 mL
Wash Solution	22 mL
Elution Buffer	12 mL
Proteinase K	12 mg
RNase	1 tube
gDNA Purification Micro Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1