Norgen Biotek™ FFPE RNA/DNA Purification Plus Kit Dx, 50 Preps

Norgen Biotek™ FFPE RNA/DNA Purification Plus Spin Column Kit Dx, 50 Preps

Norgen Biotek Corp.™

SKU:

Dx54300

$655.00

Net 30 Terms Available

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Fast and easy processing using rapid spin-column format High yields and quality of nucleic acids Separate fractionation of RNA and DNA Isolate total RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Purificati…

Fast and easy processing using rapid spin-column format
High yields and quality of nucleic acids
Separate fractionation of RNA and DNA
Isolate total RNA, from large rRNA down to microRNA (miRNA)
No phenol or chloroform extractions
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s FFPE RNA/DNA Purification Plus Kit Dx (Dx54300) provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples for subsequent in vitro diagnostic use. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of nucleic acids followed by signal detection or amplification. Any diagnostic results generated using the RNA and DNA isolated with Norgen’s FFPE RNA/DNA Purification Plus Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s FFPE RNA/DNA Purification Plus Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

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Kit Specifications
Maximum Column Binding Capacity (RNA)	35 μg
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Column Loading Volume	600 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material	4 sections <20 μM thick 10 mg of unsectioned block

Storage Conditions and Product Stability

The DNAse I and Proteinase K should be stored at -20°C upon arrival. All other solutions and kit components should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.

Component	Cat. 54300 (50 preps)
Digestion Buffer	2 x 20 mL
Binding Solution	2 x 20 mL
Enzyme Incubation Buffer A	6 mL
RNA Wash Solution	24 mL
DNA Wash Solution	38 mL
RNA Elution Solution	6 mL
DNA Elution Buffer	15 mL
Proteinase K	2 x 12 mg
DNase I	200 μL
RNA Purification Micro Columns	50
DNA Purification Micro Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	100
Product Insert	1