Norgen Biotek™ FFPE RNA Purification Spin Column Kit Dx, 50 Preps

Norgen Biotek Corp.™

SKU:

Dx25300

$455.00

Net 30 Terms Available

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CE-IVD marked in accordance with EU Directive 98/79/EC Ideal for use in in vitro diagnostic workflows Extract total RNA (including microRNA) from FFPE samples No phenol extraction step Includes DNase for optional on-column DNA removal Isolated RNA is of the highest quality and integrity Isolate a diversity of RNA species Purified RNA …

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Kit Specifications
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Loading Volume Per Spin Column	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	1-4 hours*
Maximum Amount of Starting Material	5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block
Average Yield	Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney

* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. 25300 (50 preps)
Digestion Buffer	20 mL
Binding Solution	20 mL
Enzyme Incubation Buffer	6 mL
Wash Solution	22 mL
Elution Solution	6 mL
Proteinase K	12 mg
DNase I	200 μL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1