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Norgen Biotek™ FFPE RNA Purification Spin Column Kit Dx, 50 Preps
Norgen Biotek Corp.™
$455.00
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Kit Specifications
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| Maximum Column Binding Capacity |
Up to 50 µg RNA
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| Maximum Loading Volume Per Spin Column |
650 µL
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| Size of RNA Purified |
All sizes, including small RNA (< 200 nt)
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| Time to Complete 10 Purifications |
1-4 hours*
|
| Maximum Amount of Starting Material |
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block |
| Average Yield |
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 2 years in their unopened containers.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 25300 (50 preps) |
|---|---|
| Digestion Buffer | 20 mL |
| Binding Solution | 20 mL |
| Enzyme Incubation Buffer | 6 mL |
| Wash Solution | 22 mL |
| Elution Solution | 6 mL |
| Proteinase K | 12 mg |
| DNase I | 200 μL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
