Norgen Biotek™ Leukocyte RNA Purification 96-Well Kit, 2 Plates

Norgen Biotek Corp.™

SKU:

37800

$845.00

Net 30 Terms Available

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Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer Isolate total RNA, including microRNA, without phenol Rapid and convenient spin-column format and 96-well plate for high throughput applications Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Purificatio…

Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
Isolate total RNA, including microRNA, without phenol
Rapid and convenient spin-column format and 96-well plate for high throughput applications
Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more.
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrixThese kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

This kit (37800) provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen's kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 10⁶ Leukocytes.

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Blood Input*	1 mL or 1.5 x 10⁶ Leukocytes
Standard Blood Input	150 μL
Minimum Blood Input	10 μL
Average Yield: 500 μL human blood	1.5 μg
Time to Complete 10 Purification	40 minutes

*Additional RBC Lysis Solution is required for input Volumes >150 µL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 21200 (50 preps)	Cat. 21250 (50 preps)	Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer	2 x 100 mL	2 x 1 L	2 x 90 mL
Buffer RL	30 mL	30 mL	-
Binding Solution	-	-	2 x 40 mL
Wash Solution A	38 mL	38 mL	-
Wash Solution	-	-	2 x 30 mL
Elution Solution A	6 mL	6 mL	-
Elution Solution	-	-	2 x 20 mL
Enzyme Incubation Buffer	-	6 mL	1
DNase I	-	1 Vial	-
Mini Spin Columns	50	-	-
Lysate Homogenization Column	-	50	-
Single Cell RNA Column	-	50	-
RBC Lysis 96-Well Plate	-	-	2
96-Well Filter Plate	-	-	2
Adhesive Tape	-	-	4
Collection Tubes	50	100	-
96-Well Collection Plate	-	-	2
Elution Tubes (1.7 mL)	50	50	-
96-Well Elution Plate	-	-	2
Product Insert	1	1	1