Norgen Biotek™ Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit, 10 Preps

Norgen Biotek™ Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Spin Column Kit, 10 Preps

Norgen Biotek Corp.™

SKU:

56500

$245.00

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Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution Versatile plasma/serum input ranges No phenol extractions No carrier RNA Bind and elute all RNA irrespective of size or GC content, without bias Concentrate circulating DNA, circulating and exosomal RNA, viral DNA and viral RNA i…

Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
Versatile plasma/serum input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating DNA, circulating and exosomal RNA, viral DNA and viral RNA into flexible elution volumes
Compatible with Streck Cell-Free DNA BCT® Tubes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

This kit (56500) provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating and exosomal RNA, as well as viral DNA/RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. The purified plasma/serum circulating and viral nucleic acid is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Plasma/serum cell-free circulating and viral nucleic acid has the potential to provide biomarkers for certain cancers and disease states as well as for the detection of viral infection. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders. screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

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Kit Specifications
Sample Type	Plasma/Serum
Anti-Coagulant (for Plasma)*	EDTA or Citrate
Sample Volume Range	2 to 5 mL
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	5 mL
Size of RNA Purified	All sizes including miRNA and small RNA (< 200 nt)
Size of DNA Purified	≥ 50 bp
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yield**	Variable depending on specimen

^{*This kit is suitable for the isolation of total nucleic acid (RNA and DNA) from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, especially if the primarily interest is RNA, as heparin can significantly interfere with many downstream applications such as RT-PCR. if the main interest is DNA then heparinised plasma can be used.} ^{**Please check page 7 of the product insert for average plasma/serum yields and common RNA/DNA quantification methods.}

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 56300 (50 preps)	Cat. 56400 (20 preps)	Cat. 56500 (10 preps)
Lysis Buffer A	2 x 20 mL	100 mL	1 x 130 mL 1 x 30 mL
Wash Solution A	18 mL	1 x 38 mL 1 x 18mL	38 mL
Elution Buffer F	6 mL	15 mL	15 mL
Micro Spin Columns	50	-	-
Mini Spin Columns	-	20	10
Midi Spin Columns	-	20	-
Maxi Spin Columns	-	-	10
Collection Tubes	50	20	10
Elution Tubes (1.7 mL)	50	20	10
Product Insert	1	1	1

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Norgen Biotek™ Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Spin Column Kit, 10 Preps

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Norgen Biotek™ Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Spin Column Kit, 10 Preps

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Norgen Biotek™ Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Spin Column Kit, 10 Preps

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