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Norgen Biotek™ Plasma/Serum Exosome Purification and RNA Isolation Maxi Spin Column Kit, 15 Preps

SKU:
58600
$569.00
Net 30 Terms Available

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Purification and enrichment of intact plasma/serum exosomes for functional studies Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias Versatile plasma/serum input volume No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor specia…

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Kit Specifications
Minimum Plasma/Serum Input
4 mL
Maximum Plasma/Serum Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component Cat. 58300 (50 preps) Cat. 58500 (25 preps) Cat. 58600 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 8 mL 2 x 8 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 38 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1
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