SKU: 68200
Norgen Biotek™ Plasma/Serum cfc-RNA Advanced Purification Kit, 50 Preps
Norgen Biotek Corp.™
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Kit Specifications
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Sample Volume Range |
50 to 200 μL
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Anti-coagulant (for Plasma)*
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EDTA or Citrate
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Size of RNA Purified |
All sizes, including
small RNA (< 200 nt) |
Minimum Elution Volume |
10 μL
|
Maximum Elution Volume | 25 μL |
Time to Complete 10 Purifications |
15-20 minutes
|
Average Yield** |
Variable depending on specimen
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*This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Kit Components | Cat. 55000 (50 preps) | Cat. 56100 (20 preps) | Cat. 56200 (10 preps) |
---|---|---|---|
Lysis Buffer A | 2 x 20 mL | 100 mL | 1 x 130 mL 1 x 30 mL |
Wash Solution A | 18 mL | 1 x 38 mL 1 x 18 mL |
38 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Elution Buffer F | - | 15 mL | 15 mL |
Micro Spin Columns | 50 | - | - |
Mini Spin Columns | - | 20 | 10 |
Midi Spin Columns | - | 20 | - |
Maxi Spin Columns | - | - | 10 |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
Product Insert | 1 | 1 | 1 |