SKU: 17700
Norgen Biotek™ ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit, 4 Preps
Norgen Biotek Corp.™
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Kit Specifications
|
|
Maximum Culture Volume |
100 mL
|
Yield from 100 mL of Culture |
Up to 12 mg
|
Minimum Elution Volume |
4 mL
|
Time to Process 1 Sample |
1 hour
|
Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for Binding Buffer C and Binding Buffer N. Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.
Component | Cat. 10300 (Micro - 25 preps) | Cat. 17700 (Maxi - 4 preps) |
---|---|---|
Wash Solution C | 30 mL | 130 mL |
Wash Solution N | 30 mL | 130 mL |
Binding BUffer A | 4 mL | 20 mL |
Binding Buffer N | 4 mL | 20 mL |
Elution Buffer C | 8 mL | 2 x 30 mL |
Protein Neutralizer | 4 mL | 4 mL |
Cell Lysis Reagent | 15 mL | 110 mL |
IB Solubilization Reagent | 2 mL | 50 mL |
Syringes, 1cc, slip tip | 25 | - |
Needles (Bev, 20G x 1 inch) | 25 | - |
Syringes, 10 mL, Luer-Lok™ Tip | - | 4 |
Needles (18G x 1.5 inch) | - | 4 |
Micro Spin Columns | 25 | - |
Maxi Spin Columns (filled with SiC) inserted into 50 mL collection tubes | - | 4 |
Collection Tubes | 25 | - |
Elution Tubes (1.7 mL) | 25 | - |
Elution Tubes (50 mL) | - | 4 |
Product Insert | 1 | 1 |