Norgen Biotek™ RNA/DNA/Protein Purification 96-Well Kit, 96 Preps

Norgen Biotek Corp.™

SKU:

51700

$859.00

Net 30 Terms Available

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Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample No sample splitting or need to use phenol or precipitation methods Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants Purification is based on spin column chromatography that uses Norgen’s proprietary resi…

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Kit Specifications
Binding Capacity Per Well	50 μg for RNA 20 μg for DNA 150 μg for protein
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Size of DNA Purified	≥ 30 kb
Time to Complete 10 Purifications	35 minutes
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	1 x 10⁶ cells 10 mg 100 µL 1 x 10⁹ cells 1 x 10⁸ cells 40 mg 40 mg
Average Yield* Liver (5 mg)	12.5 μg RNA 2 μg DNa 55 μg Protein

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

Component	Cat. 47700 (50 preps)	Cat. 51600 (50 preps)	Cat. 51700 (96 preps)
Buffer SKP	40 mL	40 mL	-
Lysis Buffer Q	-	-	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL	20 mL
Elution Buffer F	15 mL	15 mL	2 x 15 mL
Wash Solution C	30 mL	30 mL	60 mL
Binding Buffer A	8 mL	8 mL	8 mL
Elution Buffer C	8 mL	8 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Protein Loading Dye	2 mL	2 mL	3 x 2 mL
gDNA Purification Columns	50	-	-
gDNA Purification Micro Columns	-	50	-
gDNA Purification 96-Well Plate	-	-	1
RNA/Protein Purification Columns	50	-	-
RNA/Protein Purification Micro Columns	-	50	-
RNA/Protein Purification 96-Well Plate	-	-	1
Collection Tubes	150	150	-
Collection Plate	-	-	5
Elution Tubes (1.7 mL)	150	150	-
Elution Plate	-	-	3
Lysis Preparation Plate	-	-	2
Adhesive Tape	-	-	4
Product Insert	1	1	1