Norgen Biotek™ RNA/DNA/Protein Purification Plus Micro Spin Column Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

51600

$799.00

Net 30 Terms Available

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Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample No sample splitting or need to use phenol or precipitation methods Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants Purification is based on spin column chromatography that uses Norgen’s proprietary resi…

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Kit Specifications
Maximum Column Binding Capacity	35 μg for RNA 10 μg for DNA 100 μg for protein
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Size of DNA Purified	≥ 30 kb
Time to Complete 10 Purifications	30 minutes
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Plant / Fungi Tissues	5 x 10⁵ cells 5 mg (for selected tissues) 50 µL 25 mg
Average Yield* HeLa Cells (5 x 10⁵ cells) HeLa Cells (5 x 10⁵ cells) HeLa Cells (5 x 10⁵ cells)	RNA: 7.5 μg DNA: 1-2 μg Protein: 70 μg

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 47700 (50 preps)	Cat. 51600 (50 preps)	Cat. 51700 (96 preps)
Buffer SKP	40 mL	40 mL	-
Lysis Buffer Q	-	-	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL	20 mL
Elution Buffer F	15 mL	15 mL	2 x 15 mL
Wash Solution C	30 mL	30 mL	60 mL
Binding Buffer A	8 mL	8 mL	8 mL
Elution Buffer C	8 mL	8 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Protein Loading Dye	2 mL	2 mL	3 x 2 mL
gDNA Purification Columns	50	-	-
gDNA Purification Micro Columns	-	50	-
gDNA Purification 96-Well Plate	-	-	1
RNA/Protein Purification Columns	50	-	-
RNA/Protein Purification Micro Columns	-	50	-
RNA/Protein Purification 96-Well Plate	-	-	1
Collection Tubes	150	150	-
Collection Plate	-	-	5
Elution Tubes (1.7 mL)	150	150	-
Elution Plate	-	-	3
Lysis Preparation Plate	-	-	2
Adhesive Tape	-	-	4
Product Insert	1	1	1