Norgen Biotek™ RNA/DNA/Protein Purification Plus Spin Column Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

47700

$819.00

Net 30 Terms Available

Earn points on this purchase

Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample No sample splitting or need to use phenol or precipitation methods Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants Purification is based on spin column chromatography that uses Norgen’s proprietary resi…

Quantity:

*We accept purchase orders from private, public, educational, & government institutions

Current Stock:

Request a Quote Request a Sample

Kit Specifications
Maximum Column Binding Capacity	50 μg for RNA 20 μg for DNA 200 μg for protein
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Size of DNA Purified	≥ 30 kb
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	5 x 10⁶ cells 25 mg (for selected tissues) 100 μL 1 x 10⁹ cells 1 x 10⁸ cells 50 mg 50 mg
Time to Complete 10 Purifications	30 minutes
Average Yield: HeLa Cells (1 x 10⁶ cells) HeLa Cells (1 x 10⁶ cells) HeLa Cells (1 x 10⁶ cells)	15 μg RNA 8 μg DNA 150 μg protein

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 47700 (50 preps)	Cat. 51600 (50 preps)	Cat. 51700 (96 preps)
Buffer SKP	40 mL	40 mL	-
Lysis Buffer Q	-	-	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL	20 mL
Elution Buffer F	15 mL	15 mL	2 x 15 mL
Wash Solution C	30 mL	30 mL	60 mL
Binding Buffer A	8 mL	8 mL	8 mL
Elution Buffer C	8 mL	8 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Protein Loading Dye	2 mL	2 mL	3 x 2 mL
gDNA Purification Columns	50	-	-
gDNA Purification Micro Columns	-	50	-
gDNA Purification 96-Well Plate	-	-	1
RNA/Protein Purification Columns	50	-	-
RNA/Protein Purification Micro Columns	-	50	-
RNA/Protein Purification 96-Well Plate	-	-	1
Collection Tubes	150	150	-
Collection Plate	-	-	5
Elution Tubes (1.7 mL)	150	150	-
Elution Plate	-	-	3
Lysis Preparation Plate	-	-	2
Adhesive Tape	-	-	4
Product Insert	1	1	1