Norgen Biotek™ RNA/DNA Purification Spin Column Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

48700

$675.00

Net 30 Terms Available

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Sequentially isolate and purify total RNA and DNA from a single sample Two column system: one for DNA and one for RNA The RNA column is for the purification of total RNA including microRNA No need to split the lysate, or to use phenol or precipitation methods Rapid and efficient spin column procedure - it takes only 30 minutes Purification is b…

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Kit Specifications
Maximum Column Binding Capacity	50 μg for RNA 20 μg for DNA
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	5 x 10⁶ cells 25 mg (for most tissues)** 200 μL 1 x 10⁹ cells 1 x 10⁸ cells 50 mg 50 mg
Time to Complete 10 Purifications	30 minutes
Average Yield*: HEK 293 Cells (1 x 10⁶ cells) HEK 293 Cells (1 x 10⁶ cells) Liver (15 mg) Liver (15 mg)	10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA

*Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage.

**Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible.

Storage Conditions and Product Stability
Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48700 (50 preps)	Cat. 50300 (50 preps)
Buffer SKP	40 mL	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL
Elution Buffer F	15 mL	6 mL
RNase-Free Water	40 mL	40 mL
Proteinase K	2 x 12 mg	2 x 12 mg
gDNA Purification Columns	50	-
gDNA Purification Micro Columns	-	50
RNA Purification Columns	50	-
RNA Purification Micro Columns	-	50
Collection Tubes	100	100
Elution Tubes (1.7 mL)	100	100
Product Insert	1	1