Norgen Biotek™ Total RNA Purification Micro Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

35300

$439.00

Net 30 Terms Available

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Extract high quality & quantity total RNA including miRNA No phenol step required - isolate all RNA in one fraction Elute extracted total RNA in small volume (20 µL) Bind & elute all RNA irrespective of size or GC content, without bias. Efficiently extract small RNA irrespective of GC content Very sensitive & linear down to a …

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Kit Specifications
Maximum Column Binding Capacity	35 μg
Maximum Column Loading Volume	650 μL
Minimum Elution Volume	20 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM)	5 x 10⁵ cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 10⁵ cells
Time to Complete 10 Purifications	20 minutes
Average Yield HeLa Cells (1 x 10^{5 cells})	~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 35300 (50 preps)	Cat. 35350 (250 preps)
Buffer RL	40 mL	5 x 40 mL
Wash Solution A	38 mL	5 x 38 mL
Elution Solution A	6 mL	5 x 6 mL
Micro Spin Columns	50	250
Collection Tubes	50	250
Elution Tubes (1.7 mL)	50	250
Product Insert	1	1