Norgen Biotek™ Total RNA Purification Plus Micro Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

48500

$439.00

Net 30 Terms Available

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Extract high quality & quantity total RNA including miRNA Elute extracted total RNA in smaller volume (20 µL) No phenol step required - isolate all RNA in one fraction Genomic DNA Removal Column for efficient elimination of gDNA Bind & elute all RNA irrespective of size or GC content, without bias. Efficiently…

Extract high quality & quantity total RNA including miRNA
Elute extracted total RNA in smaller volume (20 µL)
No phenol step required - isolate all RNA in one fraction
Genomic DNA Removal Column for efficient elimination of gDNA
Bind & elute all RNA irrespective of size or GC content, without bias.
Efficiently extract small RNA irrespective of GC content
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Plus Micro Kit (48500) provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Genomic DNA is removed from the sample using a Genomic DNA Removal column, and the RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). The DNA is then captured on a Genomic DNA Removal Column. Ethanol is then added to the flowthrough of the DNA removal step, and the solution is loaded onto an RNA Purification Micro Column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The special design of the micro spin-column allows a small elution volume of as little as 20 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

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Kit Specifications
Maximum Column Binding Capacity	35 μg
Maximum Column Loading Volume	650 μL
Minimum Elution Volume	20 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, Muscle Laser-Captured Microdissection (LCM)	5 x 10⁵ cells 3 mg (for most tissues*) Up to 7.5 mL Up to 5 x 10⁵ cells
Time to Complete 10 Purifications	22 minutes
Average Yield* HeLa Cells (1 x 10⁵ cells)	1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48500 (50 preps)
Buffer RL	40 mL
Wash Solution A	38 mL
Elution Solution A	6 mL
Genomic DNA Removal Column	50
RNA Purification Micro Column	50
Collection Tubes	100
Elution Tubes (1.7 mL)	50
Product Insert	1