Norgen Biotek™ Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Spin Column Kit, 20 Preps

• Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
• Versatile urine input ranges
• No phenol extractions nor carrier RNA
• Bind and elute all RNA irrespective of size or GC content, without bias
• Concentrate circulating DNA, circulating RNA and exosomal RNA, viral DNA, viral RNA into a flexible elution volumes
• Compatible with fresh, frozen or preserved urine sample
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit (60000) provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating RNA including exosomal RNA as well as viral DNA/RNA from fresh, frozen or preserved urine samples. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. These kits are designed to isolate all sizes of cfc-DNA and cfc-RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits provide a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified nucleic Acid is of the highest integrity, and can be used in a any downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating (cfc) nucleic acids such as DNA and RNA, including exosomal RNA, in urine contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types, for monitoring the disease status as well as for the detection fetal DNA. Moreover, urine has been widely used for the detection of viral infections, including HIV, HCV and HBV.The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures, and repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acids is similar to that in urine or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit

For sample input volumes ranging from 2 mL - 10 mL.