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Norgen Biotek™ Urine Exosome Purification and RNA Isolation Midi Spin Column Kit, 25 Preps

SKU:
58700
$1,329.00
Net 30 Terms Available

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Purification and enrichment of intact urinary exosomes for functional studies Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Versatile urine input volumes No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes…

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*We accept purchase orders from private, public, educational, & government institutions
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Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component Cat. 58400 (50 preps) Cat. 58700 (25 preps) Cat. 58800 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1
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