SKU: 58400
Norgen Biotek™ Urine Exosome Purification and RNA Isolation Mini Spin Column Kit, 50 Preps
Norgen Biotek Corp.™
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Kit Specifications
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Minimum Urine Input |
2 mL
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Maximum Urine Input
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10 mL
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Size of Exosomes Purified
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40 nm - 150 nm
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Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Elution Volume | 50-100 μL |
Time to Complete 10 Purifications |
35 - 40 minutes
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Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Component | Cat. 58400 (50 preps) | Cat. 58700 (25 preps) | Cat. 58800 (15 preps) |
---|---|---|---|
Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
ExoC Buffer | 8 mL | 30 mL | 50 mL |
ExoR Buffer | 12 mL | 12 mL | 12 mL |
Lysis Buffer A | 20 mL | 20 mL | 20 mL |
Lysis Additive B | 2 mL | 2 mL | 2 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Mini Filter Spin Columns | 50 | 25 | 15 |
Mini Spin Columns | 50 | 25 | 15 |
Collection Tubes | 50 | 25 | 15 |
Elution Tubes (1.7 mL) | 100 | 50 | 30 |
Product Insert | 1 | 1 | 1 |