5-FOA [5-Fluoroorotic acid]

5-FOA, or 5-Fluoroorotic Acid, is a pyrimidine analog commonly used in molecular biology and genetic research. It functions as a selective agent by being converted into toxic 5-fluorouracil in cells that express the URA3 gene, allowing researchers to identify and select for cells lacking functional URA3 or related pathways. This makes 5-FOA particularly valuable in yeast genetics, microbial genetics, and studies involving gene deletion or counter-selection.

It is typically used in culture media to screen for mutants, study metabolic pathways, or perform genetic selections. Due to its biological activity, 5-FOA should be handled with care in a well-ventilated laboratory setting using appropriate personal protective equipment to minimize exposure.

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