Amphotericin B solution, 1 X 20 mL (A2942-20ML)

Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain. Amphotericin B solution has been used: •as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice •as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries •as a constituent of transport medium during the collection of equine corpora lutea •as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use. This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate. Amphotericin B remains active for 3 days in culture at 37°C.

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