Anti-Actin (Ab-1) Mouse mAb (JLA20), 1 X 1 ea (CP01-1EA)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence. Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution) 100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol. Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems. Chicken Positive Control All animal cell lines Following initial thaw, aliquot ascites and freeze (-20°C). Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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