Anti-AFP Mouse mAb (1G7), 100 µG (ST1673-100UG)

Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry. ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) In PBS, pH 7.4. For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution. A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein Negative Control 293T cells Following initial thaw, aliquot and freeze (-20°C). Toxicity: Regulatory Review (Z)

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