Anti-Cathepsin B (Ab-1) Mouse mAb (CA10), 100 µG (IM27L-100UG)

Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP. Immunoblotting (250 ng/ml) Immunoprecipitation (1 µg/ml) Immunofluorescence (see comments) Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size). Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152. Barret, A.J. 1987. Trends Biochem. Sci.12, 193. Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721. Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535. Epitope: within the heavy chain of mature cathepsin B Positive Control Hep G2 cells Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles. Toxicity: Standard Handling (A)

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