Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6), 100 µG (CC12-100UG)

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein. Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F) Frozen Sections (see application references) Immunoblotting (1 µg/ml) Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F) Immunoprecipitation (see application references) Neutralization Studies (see application references) Paraffin Sections (5 µg/ml, heat pre-treatment required) In 50 mM sodium phosphate buffer, 0.2% gelatin. Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems. Human Negative Control trp E (Ab-1) Toxicity: Standard Handling (A)

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