Anti-phospho-Tie2 (Ser1119) Antibody, 1 X 100 μL (ABS219)

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. Anti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB. Affinity purified Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Epitope: Phosphorylated Ser1119 Control HEK293T overexpressing Tie2 protein Stable for 1 year at 2-8°C from date of receipt. This antibody recognizes Tie2 phosphorylated at Ser1119. Evaluated by Western Blot in HEK293T overexpressing Tie2 protein.Western Blot Analysis: A 1:500 dilution detected Tie2 on 10 µg of HEK293T overexpressing Tie2 protein. ~160 kDa observed. Uniprot gives a calculated molecular weight of 126 kDa, but has been observed at ~160 kDa due to glycosolation. A non-modified specific band appears at ~52 kDa in some lysates.

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