Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, 1 g (382061-1GM)

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Werstuck, G., and Green, M.R. 1998. Science 282, 296. Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152. Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322. Tarshis, M., et al. 1994. J. Immunol. Methods168, 245. Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109. Oberhammer, F., et al. 1993. EMBO J. 12, 3679. Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41. Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939. Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188. Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24. Cell permeable: yes Precipitates in phosphate buffered solutions Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C. Toxicity: Carcinogenic / Teratogenic (D)

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