CellPro™ Hank's 1X Balanced Salt Solutions (HBSS) without calcium, magnesium, or phenol red

CellPro™ Hank’s 1X Balanced Salt Solution (HBSS) without calcium, magnesium, or phenol red is a high-purity, isotonic buffer designed to support a wide range of cell handling, washing, and preparation workflows. This cation-free, indicator-free formulation minimizes cell adhesion and aggregation while providing a stable physiological environment for cells outside of complete culture media.

By removing calcium and magnesium ions, the solution reduces cation-dependent cell–cell and cell–surface interactions, making it ideal for enzymatic dissociation, suspension preparation, and gentle cell processing. The absence of phenol red eliminates potential interference with fluorescence-based assays, imaging workflows, and sensitive biochemical reactions.

Key Features & Benefits

• Calcium- and magnesium-free formulation minimizes unwanted cell adhesion and supports efficient enzymatic dissociation.
• Phenol red–free to prevent dye interference in fluorescence, imaging, and color-sensitive assays.
• Physiologically balanced to maintain osmolarity and pH during cell handling outside of culture media.
• High-purity, low-endotoxin components for reliable performance in research workflows.
• Ready-to-use 1X solution ensures consistency and convenience.

Applications

• Preparation of single-cell suspensions
• Cell washing, rinsing, and centrifugation steps
• Use with trypsin or other dissociation enzymes
• Tissue processing and transport
• Fluorescence microscopy, flow cytometry, and color-sensitive assays
• Buffering for immunology, microbiology, and general cell biology protocols

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