Coagulation factor III/Tissue Factor Rabbit pAb, Polyclonal (A1378) | ABclonal Technology™

ABclonal Technology™ Coagulation factor III/Tissue Factor Rabbit pAb (A1378) is a polyclonal antibody raised in rabbit against F3. It is validated for WB, IF/ICC and ELISA. Confirmed reactivity includes Human, Mouse and Rat. The antibody has an IgG isotype and an observed molecular weight of 53kDa/45kDa. It is affinity purified.

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

• Host Species: Rabbit
• Isotype: IgG
• Species Reactivity: Human, Mouse, Rat
• Validated Applications: WB, IF/ICC, ELISA
• Recommended Dilution: WB,1:500 - 1:1000; IF/ICC,1:50 - 1:200; ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Purity: Affinity purification
• Observed MW: 53kDa/45kDa
• UniProt ID: P13726
• Storage: Store at -20℃. Avoid freeze / thaw cycles.; Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

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