CryoShield Prime™ Serum-Free Universal Cryopreservation Medium for for Cells and Organoids, Sterile-Filtered, 100mL

CryoShield Prime™ by CellPro is a high-performance, chemically defined cryopreservation medium designed to preserve the full functionality and integrity of a wide range of cells and tissues. Engineered for universal application, it is ideal for the long-term freezing of cell lines, stem cells, immune cells, hepatocytes, organoids, and more — all without requiring serum, proteins, or animal-origin ingredients.

This sterile-filtered, ready-to-use solution offers superior viability and plating efficiency after thawing, maintaining structural integrity across even the most sensitive cell types. Unlike conventional freezing media, CryoShield Prime™ is GMP-grade and completely free of antibiotics and unnecessary additives. It provides consistent protection against intracellular and extracellular ice formation, minimizing cellular stress and apoptosis during freezing and thawing.

Key Features

• Chemically defined, protein-free, serum-free, and animal-origin free
• Suitable for long-term storage at –80°C without liquid nitrogen
• Preserves viability and functionality across all major cell and tissue types
• Validated for organoids, NK-T cells, CAR-T cells, macrophages, MSCs, iPSCs, ESCs, and hepatocytes
• Maintains cell membrane, nuclear, and mitochondrial integrity
• Outperforms CryoStor CS10 and Stem Cellbanker in viability and post-thaw morphology
• GMP-grade, sterile-filtered, and ready to use

Validated Applications

• Organoids and brain spheroids
• NK-T and CAR-T cell therapy research
• Human PBMC-derived cells
• Hepatocytes and neuron models
• Stem cells, immune cells, epithelial lines, and fibroblasts
• Cell lines including CHO, HEK293T, Hela, MCF-7, etc.

Performance Highlights

• Higher post-thaw viability and morphology retention compared to CryoStor CS10
• Strong recovery of NK-T cells over a 14-day culture period
• Consistent cell survival across primary hepatocytes, brain organoids, and HEK293T cells

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