Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, 6 X 500 mL (D8062-6X500ML)

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system. Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used: •to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites •to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment •to culture mouse squamous cell carcinoma (SCCVII) tumor cells

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