Ficoll 400 [Polysucrose 400]

Ficoll 400, also known as Polysucrose 400, is a high-molecular-weight, branched polymer of sucrose and epichlorohydrin widely used in cell biology and molecular biology applications. It is a water-soluble, non-ionic, and highly hydrophilic compound that forms stable solutions, making it ideal for density gradient centrifugation, cell separation, and purification of blood components, lymphocytes, and other cell types.

Ficoll 400 is commonly employed in laboratory protocols to create density gradients for isolating mononuclear cells, separating subcellular components, or purifying viruses and macromolecules. Its biocompatibility, chemical stability, and low osmolality make it a reliable reagent for research involving delicate cells and biomolecules, ensuring minimal cytotoxicity and consistent experimental results.

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