Fluid D-USP 200mL Bottle, 1/Pk

Fluid D is a specialized sterile rinsing fluid prepared strictly in accordance with United States Pharmacopeia (USP) standards for sterility testing (USP <71>). Each bottle contains 200mL of 0.1% Peptone water (Fluid A) supplemented with 0.1% Polysorbate 80 (Tween 80).

This fluid is the mandatory rinsing agent when testing pharmaceutical products that contain oils, ointments, creams, or lecithin. The key ingredient, Polysorbate 80, acts as a surfactant/emulsifier. It breaks down the surface tension of fatty or viscous substances, preventing them from clogging membrane filters and ensuring that microorganisms are not trapped within oil droplets where they would be shielded from culture media.

Crucially, Fluid D is used to rinse the membrane filter after the sample has passed through. This step washes away any bacteriostatic or fungistatic residues (preservatives) that may be dissolved in the oil, preventing false-negative results. The 200mL volume is a convenient size for single-test protocols, often providing the exact volume needed to rinse two filtration canisters (100mL each) without the waste of bulk containers.

• USP Compliant: Formulated to meet specific USP <71> requirements.

• Emulsifying Action: Polysorbate 80 facilitates the filtration of oils and sticky products.

• Prevents False Negatives: Ensures preservatives are washed away from oily matrices.

• Application: Ideal for sterility testing of salves, lotions, and medical devices.

• Convenient Size: 200mL bottle minimizes waste for standard testing runs.

• Format: Sterile, ready-to-use solution.

Read Less