Human IL1 beta ELISA Kit, 96 TESTS

IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis, and inflammatory pain hypersensitivity. In this ELISA kit, an IL1-specific antibody has been pre-coated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL1-specific biotinylated detection antibody is added and followed by washing with wash buffer. Afterward, Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL1 captured in the plate.

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