Human Thioredoxin Reductase 1/TRXR1 Rabbit pAb, Polyclonal (A16631) | ABclonal Technology™

ABclonal Technology™ Human Thioredoxin Reductase 1/TRXR1 Rabbit pAb (A16631) is a polyclonal antibody raised in rabbit against TXNRD1. It is validated for WB, IHC-P, IF/ICC and ELISA. Confirmed reactivity includes Human, Mouse and Rat. The antibody has an IgG isotype and an observed molecular weight of 65kDa. It is affinity purified.

The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.

• Host Species: Rabbit
• Isotype: IgG
• Species Reactivity: Human, Mouse, Rat
• Validated Applications: WB, IHC-P, IF/ICC, ELISA
• Recommended Dilution: WB,1:500 - 1:2000; IHC-P,1:50 - 1:100; IF/ICC,1:50 - 1:200; ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Purity: Affinity purification
• Observed MW: 65kDa
• UniProt ID: Q16881
• Storage: Store at -20℃. Avoid freeze / thaw cycles.; Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

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