LB Broth (Luria low salt), 1 X 250 g (L3397-250G)

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation. LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique. Luria’s low salt LB powder provides: •Standard formulation •A budget-friendly alternative to liquid •Easy scale-up using larger package sizes 1. Suspend 15.5 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121°C. Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

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