LuminoCt® qPCR ReadyMix™, 1 X 100 reactions (L6669-100RXN)

Inclusion of JumpStart™ Taq antibody in the ReadyMix™ eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup. •Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms. •ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays. •Kit is not compatible with qPCR instruments that utilize glass capillary tubes. LuminoCt® qPCR ReadyMix™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis. It has also been used in qPCR for quantifying the RNA extracted from rhinoviruses. •Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix. •At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp. •Sigma recommends designing primers and probes with Beacon Designer software To order primers and probes from Sigma, visit www.sigma.com/oligos •Assay results in as little as 25 minutes •Designed to deliver unsurpassed accuracy, precision, and sensitivity •Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters

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