LuminoCt® SYBR® Green qPCR ReadyMix™, 1 X 500 reactions (L6544-500RXN)

LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup. Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assaysKit is not compatible with qPCR instruments that utilize glass capillary tubes LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression. •At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp •Sigma recommends designing primers and probes with Beacon Designer software •To order primers and probes from Sigma, visit: www.sigma.com/oligos Ideal for performing high-throughput, quantitative PCR applications The master mix allows consistency from one reaction to the next Reduced preparation time and reduced risk of contamination from multiple pipetting steps The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results This master mix is compatible with commercial primer sets, including TaqMan® assays

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