MagBeads FastRNA Kit for Blood, 50 PREPS

MagBeads FastRNA® Kit for Blood is a scalable and automatable (automatable versions available cat. no.117039100 & cat. no.117039200) magnetic bead-based RNA purification technology that is designed for total isolation of RNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate). RNA degradation prevention is the backbone of RNA purification. The use of our specially formulated Buffer MBL containing huge amounts of chaotropic ions omits the need of excessive homogenization and enables highly efficient lysis with the immediate inactivation of RNases, which are typically present in most biological materials. The MagBeads FastRNA® Kit for Blood simplifies the RNA purification process by eliminating the need for complicated erythrocyte lysis and isolation of leukocyte pellet, making it hassle-free and time efficient. Contaminated DNA is removed by a DNase I treatment step. Simplified dual washing steps largely remove salts, metabolites, and macromolecular cellular components. Pure RNA extracts of high yield and purity, compatible with downstream applications such as RT-qPCR amplification, are then eluted under low ionic strength conditions with RNase-free water. Figure 1: High Quality RNA Purification. MagBeads FastRNA® Kit for Blood delivers high yield and purity with consistent RNA extraction efficiency. Figure 2: Evaluation of the performance of the MagBeads FastRNA® kit for blood compared to Competitor M and Competitor MN . RNA was independently extracted (three extractions of quadruplicates each) from MagBeads FastRNA® kit for Blood (MP), Competitor M or Competitor MN. The RNA yield, purity (260/230 ratio) integrity, and RIN were assessed using spectrophotometry and Agilent Technologies. In addition, the extracted RNA was also assessed for its amplifiability using qPCR and the Ct values given by MP are earlier than Competitor M. The horizontal bars indicate the median value.  

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