Motility Indole Oranthine (MIO) Medium 5 mL Tube, 10/Pk

Motility Indole Ornithine (MIO) Medium is a semi-solid, "three-in-one" diagnostic tube used to differentiate members of the Enterobacteriaceae family. It simultaneously tests for Motility, Indole Production, and Ornithine Decarboxylase activity, streamlining the workflow for identifying enteric pathogens like Klebsiella, Enterobacter, and E. coli.

The medium is inoculated by a straight stab down the center of the tube. The results are read as follows:

1. Motility:

   • Positive: Diffusion of growth/turbidity extending outward from the stab line (cloudy medium).

   • Negative: Growth restricted to the stab line only (clear medium).

2. Ornithine Decarboxylase:

   • Positive (Purple): The organism decarboxylates ornithine, producing alkaline amines that turn the Bromocresol Purple indicator to a darker purple (often seen in the bottom 2/3 of the tube).

   • Negative (Yellow): The organism ferments glucose but lacks the enzyme, turning the acidic butt yellow.

3. Indole Production:

   • After reading the first two results, Kovac’s Reagent is added to the top of the tube.

   • Positive: A distinct red ring forms at the surface.

   • Negative: The reagent layer remains yellow/colorless.

This combination is particularly useful for differentiating Klebsiella species (typically Non-motile) from Enterobacter (typically Motile).

• Triple Test: Efficiently screens for Motility, Indole, and Ornithine in one tube.

• Workflow Efficiency: Reduces the number of tubes and media required for ID.

• Diagnostic Power: Key for separating Enterobacter, Klebsiella, and E. coli.

• Clear Indicators: Visual color changes (Purple/Yellow/Red Ring) for easy interpretation.

• Semi-Solid: Optimization of agar concentration allows for clear motility observation.

• Format: Sterile 5mL tubes in a 10-pack.

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