Norgen Biotek™ AAV Purification Kit Spin Column, 15 Preps

Norgen Biotek Corp.™

SKU:

66100

$865.00

Net 30 Terms Available

Earn points on this purchase

AAV Purification from any input – cell fraction or media fraction High AAV recovery, up to 90% No specialized equipment needed Purification from a variety of AAV serotypes (including AAV6 and AAV9) Yields highly active AAV for in vivo and in vitro experiments Purification is based on spin column chromatography that use…

Quantity:

*We accept purchase orders from private, public, educational, & government institutions

Current Stock:

Request a Quote Request a Sample

Kit Specifications
Resin Binding Capacity (total per kit)	At least 5 x 10¹⁰ AAV particles as determined by qPCR
AAV Vector Serotype	AAV6, AAV9 and others
Input Type	Cells, media
Input Volume (AAV supernatant)	1 - 33.5 mL SN per prep (500 mL SN in total)
Input Volume (AAV cell pellet)	1 mL cell pellet per prep (15 mL in total)
Time to Complete Purifications	2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 66100 (15 preps)	Cat. 63200 (20 preps)	Cat. 63300 (4-8 preps)	Cat. 63250 (1-10 preps)
Lysis Buffer S	5.5 mL	5.5 mL	5.5 mL	20 mL
DNAse I	-	2 x 25 uL	2 x 25 uL	210 μL
RNAse A	-	60 μL	60 μL	240 μL
HL-SAN Nuclease	102 μL	-	-	-
Binding Buffer A	20 mL	4 mL	4 mL	2 x 8 mL
Purification Solution C	60 mL	-	-	-
Purification Solution D	130 mL	-	-	-
Wash Solution C	2 x 130 mL	60 mL	60 mL	3 x 60 mL
Slurry E	12.5 mL	-	-	2 x 14.5 mL
Elution Buffer O	66 mL	8.5 mL	8.5 mL	66 mL
Protein Neutralizer	4 mL	4 mL	4 mL	4 mL
Spin Columns	-	20	-	-
Mini Spin Columns	-	20	-	-
Midi Spin Columns (grey contents) with Collection Tubes	-	-	8	10
Midi Spin Columns (white contents) with Collection Tubes	-	-	8	-
Maxi Spin Columns (grey contents) with Collection Tubes	-	-	-	10
Maxi Spin Columns (white contents) with Collection Tubes	-	-	-	10
Collection Tubes	-	40	-	-
Elution tubes (1.7 mL)	50	20	-	-
Midi Elution tubes (15 mL)	-	-	8	10
Maxi Elution tubes (50 mL)	-	-	-	10
Product Insert	1	1	1	1