Norgen Biotek™ AAV Purification Midi Kit, 4 Preps

Norgen Biotek Corp.™

SKU:

63300

$365.00

Net 30 Terms Available

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AAV Purification from any input – cell fraction or media fraction High AAV recovery, up to 90% No specialized equipment needed Purification from a variety of AAV serotypes (including AAV6 and AAV9) Yields highly active AAV for in vivo and in vitro experiments Purification is based on spin column chromatography that use…

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Kit Specifications
Column Binding Capacity	At least 1 x 10¹⁰ AAV particles as determined by qPCR
AAV Vector Serotype	Any
Average Recovery	> 80%
Input Type	Cells, media, or mixed
Input Volume	8 mL - 45 mL
Minimum Elution Volume	1 mL
Time to Complete 4 Purifications	2 - 2.5 hours

Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.

Component	Cat. 66100 (15 preps)	Cat. 63200 (20 preps)	Cat. 63300 (4-8 preps)	Cat. 63250 (1-10 preps)
Lysis Buffer S	5.5 mL	5.5 mL	5.5 mL	20 mL
DNAse I	-	2 x 25 uL	2 x 25 uL	210 μL
RNAse A	-	60 μL	60 μL	240 μL
HL-SAN Nuclease	102 μL	-	-	-
Binding Buffer A	20 mL	4 mL	4 mL	2 x 8 mL
Purification Solution C	60 mL	-	-	-
Purification Solution D	130 mL	-	-	-
Wash Solution C	2 x 130 mL	60 mL	60 mL	3 x 60 mL
Slurry E	12.5 mL	-	-	2 x 14.5 mL
Elution Buffer O	66 mL	8.5 mL	8.5 mL	66 mL
Protein Neutralizer	4 mL	4 mL	4 mL	4 mL
Spin Columns	-	20	-	-
Mini Spin Columns	-	20	-	-
Midi Spin Columns (grey contents) with Collection Tubes	-	-	8	10
Midi Spin Columns (white contents) with Collection Tubes	-	-	8	-
Maxi Spin Columns (grey contents) with Collection Tubes	-	-	-	10
Maxi Spin Columns (white contents) with Collection Tubes	-	-	-	10
Collection Tubes	-	40	-	-
Elution tubes (1.7 mL)	50	20	-	-
Midi Elution tubes (15 mL)	-	-	8	10
Maxi Elution tubes (50 mL)	-	-	-	10
Product Insert	1	1	1	1