Norgen Biotek™ Adenovirus Purification Kit, 15 Preps

Norgen Biotek Corp.™

SKU:

67600

$839.00

Net 30 Terms Available

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Adenovirus Purification from any input – cell fraction or media fraction Rapid purification within 2 to 4.5 hours No specialized equipment needed (ultracentrifuge not required) Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep Purify adenovirus cell pellet from 1 mL of input per prep Up to 25X sample…

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Kit Specifications
Resin Binding Capacity (total per kit)	At least 3x10¹¹ adenovirus particles as determined by qPCR At least 3x10⁸ transducing units as determined by transduction assay
Input type	Cells, media
Input volume (Supernatant)	1 - 33.5 mL SN per prep (500 mL SN in total)
Input Volume (Cell pellet)	1 mL cell pellet per prep (15 mL in total)
Minimum elution volume	1.3 mL per prep
Time to complete purification	2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 67600 (15 preps)
Lysis Buffer S	5.5 mL
HL-SAN Nuclease	102 µL
Binding Buffer A	20 mL
Purification Solution C	60 mL
Purification Solution D	130 mL
Wash Solution C	2 x 130 mL
Slurry E	12.5 mL
Elution Buffer P (store at 4°C)	66 mL
Protein Neutralizer	4 mL
Elution tubes (1.7 mL)	50
Product Insert	1