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Norgen Biotek™ Cells and Tissue DNA Isolation Spin Column Kit, 50 Preps

SKU:
53100
$195.00
Net 30 Terms Available

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Fast, reproducible and easy processing of samples High yield and high quality genomic DNA with no RNA or protein contamination DNA ready for any application including PCR, qPCR, genotyping and more Norgen’s Cells and Tissue DNA Isolation Kit (53100) are designed for the rapid preparation of genomic DNA from cultured cells as well as var…

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Kit Specifications
Maximum Input

20 mg of animal tissue
Up to 150 μL of viral suspension
3 x 106 cells

Column Binding Capacity > 50 μg
Average Yield 8 μg (from 1 x 106 HeLa Cells)
10 μg (from 10 mg kidney)
Elution Volume 50 - 200 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Format Spin Column
Time to Complete 10 Purifications 30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component Cat. 53100 (50 preps) Cat. 57300 (50 preps) Cat. 59100 (50 preps) Cat. 62500 (192 preps)
Lysis Buffer B 20 mL 20 mL 20 mL 1 x 40 mL
1 x 20 mL
Solution WN 18 mL 18 mL 18 mL 55 mL
Wash Solution A 18 mL 18 mL - -
Elution Buffer B 30 mL 8 mL 15 mL 1 x 30 mL
1 x 15 mL
Proteinase K 1.2 mL 1.2 mL 1.2 mL -
Proteinase K in Sotrage Buffer - - - 4 mL
Magnetic Bead Suspension - - 2 x 1.1 mL 8.5 mL
96-Well Plate - - - 2
Spin Columns 50 - - -
Micro Spin Columns - 50 - -
Collection Tubes 50 50 - -
Elution Tubes (1.7 mL) 50 50 50 -
96-Well Elution Plate - - - 2
Adhesive Tape - - - 2
Product Insert 1 1 1 1
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