Norgen Biotek™ Cells and Tissue DNA Isolation Spin Column Kit, 50 Preps
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Kit Specifications
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| Maximum Input |
20 mg of animal tissue |
| Column Binding Capacity | > 50 μg |
| Average Yield | 8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney) |
| Elution Volume | 50 - 200 μL |
| Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
| Format | Spin Column |
| Time to Complete 10 Purifications | 30 min (cells) and 90 min (tissue) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 18 mL | - | - |
| Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | - |
| Proteinase K in Sotrage Buffer | - | - | - | 4 mL |
| Magnetic Bead Suspension | - | - | 2 x 1.1 mL | 8.5 mL |
| 96-Well Plate | - | - | - | 2 |
| Spin Columns | 50 | - | - | - |
| Micro Spin Columns | - | 50 | - | - |
| Collection Tubes | 50 | 50 | - | - |
| Elution Tubes (1.7 mL) | 50 | 50 | 50 | - |
| 96-Well Elution Plate | - | - | - | 2 |
| Adhesive Tape | - | - | - | 2 |
| Product Insert | 1 | 1 | 1 | 1 |