Norgen Biotek™ FFPE RNA Purification Spin Column Kit, 50 Preps

Norgen Biotek Corp.™

SKU:

25300

$435.00

Net 30 Terms Available

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Extract total RNA (including microRNA) from FFPE samples No phenol extraction step Includes DNase for optional on-column DNA removal Isolated RNA is of the highest quality and integrity Isolate a diversity of RNA species Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Purification is base…

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Kit Specifications
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Loading Volume Per Spin Column	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	1-4 hours*
Maximum Amount of Starting Material	5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block
Average Yield	Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney

* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.

Component	Cat. 25300 (50 preps)	Cat. 25400 (2 x 96 preps)
Digestion Buffer A	25 mL	2 x 25 mL
Buffer RL	30 mL	2 x 30 mL
Enzyme Incubation Buffer	6 mL	2 x 6 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 20 mL
Proteinase K	12 mg	2 x 20 mg
DNase I	1 vial	2 x 500μL
Micro Spin Columns	50	-
96-Well Incubation Plate	-	2
96-Well Plate	-	2
Adhesive Tape	-	8
Collection Tubes	50	-
96-Well Collection Plate	-	2
Elution Tubes (1.7 mL)	50	-
96-Well Elution Plate	-	2
Product Insert	1	1